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size exclusion chromatography  (Bio-Rad)


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    Structured Review

    Bio-Rad size exclusion chromatography
    Size Exclusion Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+spin+6+chromatography+columns/Micro+Bio-Spin+P-6+Gel+Columns/us12612433-390-14-22
    Average 96 stars, based on 644 article reviews
    size exclusion chromatography - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Highly clustered de novo frameshift variants in the neuronal splicing factor NOVA2 result in a specific abnormal C terminal part and cause a severe form of intellectual disability with autistic features
    Article Snippet: After transcription, 1 unit of DNase I (Invitrogen, Carlsbad, CA) was added, and the sample was incubated for additional 30 min at 37°C. .. Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. ..

    Article Title: De Novo Frameshift Variants in the Neuronal Splicing Factor NOVA2 Result in a Common C-Terminal Extension and Cause a Severe Form of Neurodevelopmental Disorder
    Article Snippet: After transcription, 1 unit of DNase I (Invitrogen) was added, and the sample was incubated for additional 30 min at 37°C. .. Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. ..

    Chromatography:

    Article Title: Highly clustered de novo frameshift variants in the neuronal splicing factor NOVA2 result in a specific abnormal C terminal part and cause a severe form of intellectual disability with autistic features
    Article Snippet: After transcription, 1 unit of DNase I (Invitrogen, Carlsbad, CA) was added, and the sample was incubated for additional 30 min at 37°C. .. Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. ..

    Article Title: De Novo Frameshift Variants in the Neuronal Splicing Factor NOVA2 Result in a Common C-Terminal Extension and Cause a Severe Form of Neurodevelopmental Disorder
    Article Snippet: After transcription, 1 unit of DNase I (Invitrogen) was added, and the sample was incubated for additional 30 min at 37°C. .. Transcribed RNAs were then purified by micro Bio-Spin 6 chromatography columns (Bio-rad) according to the manufacturer’s instructions. ..

    Article Title: Molecular sleds and uses thereof
    Article Snippet: .. Excess reagents were removed from the labeled sample by passage through Bio-Spin 6 Chromatography columns (Bio-Rad; Hercules, Calif.) equilibrated in the labeling buffer. ..

    Article Title: Characterization of Butyrylcholinesterase from Porcine Milk
    Article Snippet: .. Bio-Spin® 6 chromatography columns and Biogel A 1.5 m column were from Bio-Rad Laboratories (Hercules, CA). .. Enzyme-linked immunosorbent assays identified 3 anti-HuBChE monoclonal antibodies that recognize PoBChE.

    Article Title: Compositions and methods for treating viral infections
    Article Snippet: .. Counterscreens The ability of the compounds of the present invention to inhibit human DNA polymerases are measured in the following assays. a. Inhibition of Human DNA Polymerases Alpha and Beta: Reaction Conditions: 50 μL reaction volume Reaction Buffer Components: 20 mM Tris-HCl, pH 7.5 200 μg/mL bovine serum albumin 100 mM KCl2 mM β-mercaptoethanol 10 mM MgCl2 1.6 μM dA, dG, dC, dTTP α33P-dATp Enzyme and Template: 0.05 mg/mL gapped fish sperm DNA template 0.01 U/μL DNA polymerase a or B Preparation of Gapped Fish Sperm DNA Template: Add 5 μL 1M MgCl2 to 500 μL activated fish sperm DNA (USB 70076); Warm to 37° C. and add 30 μL of 65 U/μL of exonuclease III (GibcoBRL 18013-011); Incubate 5 min at 37° C.; Terminate reaction by heating to 65° C. for 10 min; Load 50-100 μL aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5; Elute by centrifugation at 1,000.times.g for 4 min; Pool eluate and measure absorbance at 260 nm to determine concentration. ..

    Article Title: Proline 285 is integral for the reactivation of organophosphate-inhibited human butyrylcholinesterase by 2-PAM.
    Article Snippet: .. Bio-Spin® 6 chromatography columns were purchased from Bio-Rad Laboratories, Hercules, CA. .. BChE activity was measured by the Ellman method [14] in 50 mM sodium phosphate buffer, pH 8.0, at 22 °C, in the presence of 1.0 mM DTNB and 1.0 mM BTC as the substrate.

    Labeling:

    Article Title: Molecular sleds and uses thereof
    Article Snippet: .. Excess reagents were removed from the labeled sample by passage through Bio-Spin 6 Chromatography columns (Bio-Rad; Hercules, Calif.) equilibrated in the labeling buffer. ..

    Inhibition:

    Article Title: Compositions and methods for treating viral infections
    Article Snippet: .. Counterscreens The ability of the compounds of the present invention to inhibit human DNA polymerases are measured in the following assays. a. Inhibition of Human DNA Polymerases Alpha and Beta: Reaction Conditions: 50 μL reaction volume Reaction Buffer Components: 20 mM Tris-HCl, pH 7.5 200 μg/mL bovine serum albumin 100 mM KCl2 mM β-mercaptoethanol 10 mM MgCl2 1.6 μM dA, dG, dC, dTTP α33P-dATp Enzyme and Template: 0.05 mg/mL gapped fish sperm DNA template 0.01 U/μL DNA polymerase a or B Preparation of Gapped Fish Sperm DNA Template: Add 5 μL 1M MgCl2 to 500 μL activated fish sperm DNA (USB 70076); Warm to 37° C. and add 30 μL of 65 U/μL of exonuclease III (GibcoBRL 18013-011); Incubate 5 min at 37° C.; Terminate reaction by heating to 65° C. for 10 min; Load 50-100 μL aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5; Elute by centrifugation at 1,000.times.g for 4 min; Pool eluate and measure absorbance at 260 nm to determine concentration. ..

    Centrifugation:

    Article Title: Compositions and methods for treating viral infections
    Article Snippet: .. Counterscreens The ability of the compounds of the present invention to inhibit human DNA polymerases are measured in the following assays. a. Inhibition of Human DNA Polymerases Alpha and Beta: Reaction Conditions: 50 μL reaction volume Reaction Buffer Components: 20 mM Tris-HCl, pH 7.5 200 μg/mL bovine serum albumin 100 mM KCl2 mM β-mercaptoethanol 10 mM MgCl2 1.6 μM dA, dG, dC, dTTP α33P-dATp Enzyme and Template: 0.05 mg/mL gapped fish sperm DNA template 0.01 U/μL DNA polymerase a or B Preparation of Gapped Fish Sperm DNA Template: Add 5 μL 1M MgCl2 to 500 μL activated fish sperm DNA (USB 70076); Warm to 37° C. and add 30 μL of 65 U/μL of exonuclease III (GibcoBRL 18013-011); Incubate 5 min at 37° C.; Terminate reaction by heating to 65° C. for 10 min; Load 50-100 μL aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5; Elute by centrifugation at 1,000.times.g for 4 min; Pool eluate and measure absorbance at 260 nm to determine concentration. ..

    Concentration Assay:

    Article Title: Compositions and methods for treating viral infections
    Article Snippet: .. Counterscreens The ability of the compounds of the present invention to inhibit human DNA polymerases are measured in the following assays. a. Inhibition of Human DNA Polymerases Alpha and Beta: Reaction Conditions: 50 μL reaction volume Reaction Buffer Components: 20 mM Tris-HCl, pH 7.5 200 μg/mL bovine serum albumin 100 mM KCl2 mM β-mercaptoethanol 10 mM MgCl2 1.6 μM dA, dG, dC, dTTP α33P-dATp Enzyme and Template: 0.05 mg/mL gapped fish sperm DNA template 0.01 U/μL DNA polymerase a or B Preparation of Gapped Fish Sperm DNA Template: Add 5 μL 1M MgCl2 to 500 μL activated fish sperm DNA (USB 70076); Warm to 37° C. and add 30 μL of 65 U/μL of exonuclease III (GibcoBRL 18013-011); Incubate 5 min at 37° C.; Terminate reaction by heating to 65° C. for 10 min; Load 50-100 μL aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5; Elute by centrifugation at 1,000.times.g for 4 min; Pool eluate and measure absorbance at 260 nm to determine concentration. ..



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